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PhD Student - Assay Development for the AHR Signature in Dermatology
Essen, Düsseldorf
Aktualität: 16.03.2023


16.03.2023, Deutsches Krebsforschungszentrum (DKFZ)
Essen, Düsseldorf
PhD Student - Assay Development for the AHR Signature in Dermatology
Cell culture: thaw, culture, split, freeze cells; functional in vitro analyses Molecular biology / protein chemistry: PCR, RT-PCR, qRT-PCR, DNA and RNA isolation, protein isolation, Western blots Primary animal experimental research on tumor metastasis in a translational research project that also includes human experimental issues in patients with malignant melanoma Immunohistology / immunofluorescence: paraffin / cryoembedding, preparation of slides on microtome, photography of stains on microscope, processing of images on computer Molecular and flow cytometric study of tumors Planning and execution of metabolomics and lipidomics measurements, among others, for the characterization of metastases of malignant melanoma Development of new methods for the characterization of metabolites and lipids using modern multidimensional methods (e.g. LC-LC-MS) Planning and execution of cell culture experiments Statistical evaluation of results Documentation and evaluation of research results as well as preparation of publications, lectures, posters Preparation of the dissertation within the PhD project
University degree (Master) in chemistry, food chemistry or biochemistry of at least 8 semesters Animal experimental experience (breeding / keeping immunodeficient and immunocompetent mouse lines) and knowledge of basic immunological and molecular biology techniques Proficiency in complex multicolor flow cytometry experiments and cell sorting Profound knowledge of analytical chemistry (especially in the fields of LC, GC and MS) Knowledge of metabolite and lipid analysis as well as bioinformatics is desirable High degree of independence, motivation and interest in the development of modern methods and their application in cancer research Among candidates of equal aptitude and qualifications, a person with disabilities will be given preference.




PhD Student - Assay Development for the AHR Signature in Dermatology

Essen, Düsseldorf