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Externally Funded Doctoral Student (m/f/d) - Epigenetic Regulation of Pancreatic Cancer Cell Plasticity (Johnsen group)
Stuttgart
Aktualität: 29.09.2024

Anzeigeninhalt:

29.09.2024, Robert-Bosch-Krankenhaus GmbH
Stuttgart
Externally Funded Doctoral Student (m/f/d) - Epigenetic Regulation of Pancreatic Cancer Cell Plasticity (Johnsen group)
Aufgaben:
Job Description Utilize modern sequencing-based molecular biology approaches (RNA-seq, ChIP-seq, HiChIP, Hi-C, PRO-seq, etc.) to test hypothesis and examine epigenetic and gene regulatory mechanisms controlling pancreatic tumor cell identity and therapy response. Bioinformatic analysis and interpretation of data followed by hypothesis testing and generation. Perform multiplex immunofluorescence staining to confirm gene regulatory mechanisms in primary tumor tissue using multispectral imaging with an Akoya PhenoImager. Perform tissue-based preparation of histological samples (FFPE tumor material) for single cell RNA-sequencing using the 10X Genomics technology. Perform advanced cell culture experiments to examine the effects of drug treatment in various translational model systems. Participation in internal or external research collaborations. Documentation of work procedures, data acquisition and analyses
Qualifikationen:
Applicants must have completed a Master of Science in a related field (biology, molecular biology, biotechnology or similar) The applicant must have already secured or be able to secure external funding for their position (e.g., doctoral fellowship from DAAD, China Scholarship Council, or similar) Experience with cell culture, transfections, etc. Extensive molecular biology experience (real-time PCR, Western blot, etc.) Strong interest in learning and gaining proficiency in bioinformatic analyses Strong work ethic and high level of self-motivation Independent, goal-oriented and structured way of working and ability to work in a team Confident written and spoken English skills

Berufsfeld

Bundesland

Standorte

Externally Funded Doctoral Student (m­­/­­f­­/­­d) - Epigenetic Regulation of Pancreatic Cancer Cell Plasticity (Johnsen group)

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Stuttgart